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Inserir no projetoP3031760Sistema de treinamento em procedimento de separação cromatográfica: Cromatografia gasosa com Cobra4


Chromatographic procedures allow a separation of substance mixtures with the aid of a stationary separation phase and a mobile phase. In gas chromatography the mobile phase is a gas. The mobile phase, to which the mixture to be separated is added, transports the substance mixture through the separation column at a constant flow rate. Interactions occur between the mobile phase and the stationary phase. The establishment of equilibria between the stationary phase and the different substances (distribution equilibria, adsorption-desorption equilibria) results in different migration rates of the individual components. At the end of the column there is a detector in the form of a thermal conductivity cell, which can detect the different substances on the basis of their differing thermal conductivities. The detector signal is recorded as a funtion of time. The different thermal conductivities of the carrier gas and the substance cause temperature alterations in the electrically heated temperature sensor, which is located in a Wheatstone bridge circuit. The resulting electrical signal is recorded by a plotter as a function of time (chromatogram).


  1. Determine the retention times of different gases and perform a chromatographic material separation of a mixture of butane gases.
  2. Separate and identify the components of a two-component mixture consisting of ethanol and ethyl acetate chromatographically.


What you can learn about

  • Chromatography
  • Chromatogram
  • Multiplicative distribution
  • Nernst's law of distribution (number of theoretical trays)
  • Thermal conductivity detector


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